Impact of acidifiers on the intestinal microbiota and zootechnical indicators in growing fattening pigs
DOI:
https://doi.org/10.31279/2949-4796-2026-16-1-27-40Keywords:
intestinal microbiota, pigs, fattening, acidifiersAbstract
Introduction. Currently, the search for technologies that can improve the survival, productivity, and immunity of livestock and prevent the development of antibiotic resistance in the microbiota is a priority. Antibiotics, which destroy normal flora and develop dysbiosis, contribute to the spread of resistance, and therefore pose a threat to human health, are the primary preventative measures. Acidifiers, which affect the intestinal microbiota, metabolism, and productive performance of animals, hold great promise for solving this problem.
Aim. To study the effect of acidifiers on the intestinal microbiota and zootechnical performance in fattening pigs.
Materials and methods. The study involved fattening pigs, whose diets were supplemented with acidifiers at 78 days of age. The experiment was conducted at the Priozerny Pig Complex (Leningrad Oblast, Russia). For the experiment, three groups of animals were formed: a control group fed a standard diet, experimental group 1 fed the new acidifier MetaBak, and experimental group 2 fed the acidifier Productiv Acid SE. The experiment lasted two weeks, after which intestinal contents were sampled for microbiota analysis using NGS sequencing. At the end of the fattening period, zootechnical parameters, including live weight, livability, and feed conversion, were assessed.
Results. A positive effect of both acidifiers on the parameters studied was established. The best results in livability and live weight gain were achieved with the use of the new acidifier MetaBak in the diets: the average daily gain was 953 g (+4.27%), and livability was 99.07%. The use of both products contributed to the normalization of the intestinal microbiota, increasing the content of microorganisms associated with metabolic properties. The lowest levels of opportunistic and undesirable bacteria were detected with the use of the new MetaBak acidifier, which resulted in a 2.25-fold reduction in the number of Burkholderiales, a 1.4-fold reduction in Campylobacterales, and a complete elimination of Mycoplasma from the intestinal microbial community.
Conclusions. Based on the experimental results, it can be concluded that the studied acidifiers in fattening pig diets normalize the intestinal microbiota and improve zootechnical performance.
To cite: Sklyarov S.P., Krylov R.A., Ilina L.A., Savenkov K.S., Ponomareva E.S., Zaikin V.A., Laptev G.Y. Impact of acidifiers on the intestinal microbiota and zootechnical indicators in growing fattening pigs. Agrarian Bulletin of the North Caucasus. 2026;16(1):27-40. (In Russ.) https://doi.org/10.31279/2949-4796-2026-16-1-27-40
References
Korolev A.V. "Bio-Halkvinol" instead of feed antibiotics. Pigbreeding. 2018;1:21-23. (In Russ.).
Kalyuga V.V., Trifanov A.V., Bazykin V.I. Justification of a five-phase stress-free method of keeping pigs on small farms. Pigbreeding. 2018; 8:17-20. (In Russ.).
Quan. J., Wu Z., Ye Y. et al. Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency. Frontiers in microbiology. 2020;11:32. https://doi.org/10.3389/fmicb.2020.00032
Liu. Y., Espinosa C.D., Abelilla J.J., et al. Non-antibiotic feed additives in diets for pigs: A review. Animal Nutrition. 2018;4:113–125. https://doi.org/10.1016/j.aninu.2018.01.007
Cromwell. G.L. Why and how antibiotics are used in swine production. Animal Biotechnology. 2002;13:7–27. https://doi.org/10.1081/ABIO-120005767
Han. Y.S., Tang C.H., Zhao Q.Y., et al. Effects of dietary supplementation with combinations of organic and medium chain fatty acids as replacements for chlortetracycline on growth performance, serum immunity, and faecal microbiota of weaned piglets. Livestock Science. 2018;216:210–218. https://doi.org/10.1016/j.livsci.2018.08.013
Kuppusamy S., Kakarlab D., Venkateswarluc K., et al. Agriculture, Ecosystems and Environment Veterinary antibiotics (VAs) contamination as a global agro-ecological issue: A critical view. Agriculture Ecosystems & Environment. 2018;257:47–59. https://doi.org/10.1016/j.agee.2018.01.026
Long S.F., Xu Y.T., Pan L., et al. Mixed organic acids as antibiotic substitutes improve performance, serum immunity, intestinal morphology and microbiota for weaned piglets. Animal Feed Science and Technology. 2018;235:23–32. https://doi.org/10.1016/j.anifeedsci.2017.08.018
Holman D.B., Kommadath A., Tingley J.P., Abbott D.W. Novel Insights into the Pig Gut Microbiome Using Metagenome-Assembled Genomes. Microbiology Spectrum. 2022;10(4):e0238022. https://doi.org/10.1128/spectrum.02380-22
Yi. L., Cheng. W., Song. G., et al. Integrating Machine Learning to Identify Key Microbiota of Gut Community Changes Across Different Stages in Dahe Black Pigs. Microorganisms. 2025;13(9):2038. https://doi.org/10.3390/microorganisms13092038
Koloskova E.M., Ostrenko K.S., Ezersky V.A., et al. Study of the scar microbiome in sheep using molecular genetic methods (review). Problems of biology of productive animals. 2020;4:5-26. https://doi.org/10.25687/1996-6733
Söllinger A., Tveit A.T., Poulsen M., et al. Holistic assessment of rumen microbiome dynamics through quantitative metatranscriptomics reveals multifunctional redundancy during key steps of anaerobic feed degradation. Msystems. 2018;3:e00038-18. https://doi.org/10.1128/mSystems.00038-18
Wu I.W., Lee C.C., Hsu H.J., et al. Compositional and functional adaptations of intestinal microbiota and related metabolites in CKD patients receiving dietary protein restriction. Nutrients. 2020;12:nu12092799. https://doi.org/10.3390/nu12092799
Chen L., Shen Y., Wang C., et al. Megasphaera elsdenii lactate degradation pattern shifts in rumen acidosis models. Frontiers in Microbiology. 2019;10:162. https://doi.org/10.3389/fmicb.2019.00162
Cabral L. d. S., Weimer P. J. Megasphaera elsdenii: Its Role in Ruminant Nutrition and Its Potential Industrial Application for Organic Acid Biosynthesis. Microorganisms. 2024;12(1):219. https://doi.org/10.3390/microorganisms12010219
Rhouma M., Braley C., Thériault W., et al. Evolution of Pig Fecal Microbiota Composition and Diversity in Response to Enterotoxigenic Escherichia coli Infection and Colistin Treatment in Weaned Piglets. Microorganisms. 2021;9(7):1459. https://doi.org/10.3390/microorganisms9071459
Rodrigues L.M., Neto T.O.D.A.L., Garbossa C.A.P., et al. Benzoic acid combined with essential oils can be an alternative to the use of antibiotic growth promoters for piglets challenged with E. coli F4. Animals. 2020;10:1978. https://doi.org/10.3390/ani10111978
Montoya D., D’angelo M., Martín-Orúe S.M., Rodríguez-Sorrento, A., et al. Effectiveness of two plant-based in-feed additives against an Escherichia coli F4 oral challenge in weaned piglets. Animals. 2021;11:2024. https://doi.org/10.3390/ani11072024
Samanta A.K., Gali J.M., Dutta T.K., et al. Effect of dietary phytobiotic mixture on growth performance, nutrient utilization, and immunity in weaned piglets. Tropical animal health and production. 2021;53:459. https://doi.org/10.1007/s11250-021-02910-0
More G., Lall N., Hussein A., Tshikalange T.E. Antimicrobial constituents of Artemisia afra Jacq. ex Willd. against periodontal pathogens. Evidence-Based Complementary and Alternative Medicine. 2012;252758. https://doi.org/10.1155/2012/252758
Moyo P., Kunyane P., Selepe M.A., et al. Bioassay-Guided Isolation and Identification of Gametocytocidal Compounds from Artemisia afra (Asteraceae). Malaria Journal. 2019;18:65. https://doi.org/10.1186/s12936-019-2694-1
Tariq S., Wani S., Rasool W., et al. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microbial Pathogenesis. 2019;134:103580. https://doi.org/10.1016/j.micpath.2019.103580
Betancour-Murillo C.L., Aguilar-Marin S.B., Jovel J. Prevotella: A key player in ruminal metabolism. Microorganisms. 2022;11:1. https://doi.org/10.3390/microorganisms11010001
Lückstädt C., Wylie L., Remmer R., et al. Organic Acids in Animal Nutrition, Anitox: Lawrenceville, GA, USA, 2014.
Mounir M., Ibijbijen A., Farih K., et al. Their Antioxidant Properties, Mechanisms, and Benefits on Human and Animal Health: A Narrative Review. Biomolecules. 2022;12: 1443. https://doi.org/10.3390/biom12101443
Grilli E., Messina M.R., Tedeschi M., Piva A. Feeding a microencapsulated blend of organic acids and nature identical compounds to weaning pigs improved growth performance and intestinal metabolism. Livestock Science. 2010;133:173–175. https://doi.org/10.5187/jast.2021.e16
Ahmed S.T., Hwang J.A., Hoon J., et al. Comparison of Single and Blend Acidifiers as Alternative to Antibiotics on Growth Performance, Faecal Microflora, and Humoral Immunity in Weaned Piglets. Asian-Australas. Journal of Animal Science. 2014;27:93–100. https://doi.org/10.5713/ajas.2013.13411
De Lange C.F.M., Pluske J., Gong J., Nyachoti C.M. Strategic use of feed ingrédients and feed additives to stimulate gut health and development in young pigs. Livestock Science. 2010;134:124–134. https://doi.org/10.1016/j.livsci.2010.06.117
Papatsiros V.G., Christodoulopoulos G., Filippopoulos L.C. The use of organic acids in monogastric animals (swine and rabbits). Journal of Cell and Animal Biology. 2012;6:154–159. https://doi.org/10.5897/JCAB11.081
Li S., Zheng J., Deng K., et al. Supplementation with organic acids showing different effects on growth performance, gut morphology, and microbiota of weaned pigs fed with highly or less digestible diets. Journal of Animal Science. 2018;96:3302–3318. https://doi.org/10.1093/jas/sky197
Fernandes A., Jobby R. Bacteriocins from lactic acid bacteria and their potential clinical applications. Applied Biochemistry and Biotechnology. 2022;194:4377–4399. https://doi.org/10.1007/s12010-022-03870-3
Tang H.W., Phapugrangkul P., Fauzi H.M., Tan J.S. Lactic Acid Bacteria Bacteriocin, an Antimicrobial Peptide Effective Against Multidrug Resistance: A Comprehensive Review. International Journal of Peptide Research and Therapeutics. 2021;28:14. https://doi.org/10.3390/ijms26094124
Umair M., Jabbar S., Zhaoxin L., et al. Probiotic-Based Bacteriocin: Immunity Supplementation Against Viruses. An Updated Review. Frontiers in Microbiology. 2022;13: 876058. https://doi.org/10.3389/fmicb.2022.876058
Liu X., Qian M., Shen Y., et al. An high-through put sequencing approach to the preliminary analysis of bacterial communities associated with changes in amino acid nitrogen, organic acid and reducing sugar contents during soy sauce fermentation. Food Chemistry. 2021,349:129131. https://doi.org/10.1016/j.foodchem.2021.129131
Tugnoli B., Giovagnoni G., Piva A., Grilli E. From acidifiers to intestinal health enhancer: How organic acids can improve growth efficiency of pigs. Animals. 2020,10:134. https://doi.org/10.3390/ani10010134
Soler C., Goossens T., Bermejo A., et al. Digestive microbiota is different in pigs receiving antimicrobials or a feed additive during the nursery period. PLoS ONE. 2018,13:e0197353. https://doi.org/10.1371/journal.pone.0197353
Franz C., Baser K.H.C., Windisch W. Essential oils and aromatic plants in animal feeding—A European perspective. A review. Flavour and Fragrance Journal. 2010,25:327–340. https://doi.org/10.1002/ffj.1967
Lee J., Kim J.W., Hall H., Nyachoti C.M. Effect of dietary organic acids supplementation on growth performance, nutrient digestibility, and gut morphology in weaned pigs. Canadian Journal of Animal Science. 2021,102:255–265. https://doi.org/10.1139/CJAS-2021-0080
Huang Y., Yoo J.S., Kim H.J., et al. Effects of Dietary Supplementation with Blended Essential Oils on Growth Performance, Nutrient Digestibility, Blood Profiles and Fecal Characteristics in Weanling Pigs. Asian-Australasian Journal of Animal Sciences. 2010,23:607–613. https://doi.org/10.5713/ajas.2010.80120
Wang J.P., Lee J.H., Yoo J.S., et al. Effects of phenyllactic acid on growth performance, intestinal microbiota, relative organ weight, blood characteristics, and meat quality of broiler chicks. Poultry Science. 2010,89:1549–1555. https://doi.org/10.3382/ps.2009-00235
Khan S.H., Iqbal J. Recent advances in the role of organic acids in poultry nutrition. Journal of Applied Animal Research. 2016,44:359–369. https://doi.org/10.1080/09712119.2015.1079527
Duarte M.E., Kim S.W. Phytobiotics from Oregano Extracts Enhance the Intestinal Health and Growth Performance of Pigs. Antioxidants. 2022;11:2066. https://doi.org/10.3390/antiox11102066
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Copyright (c) 2026 Sklyarov S.P., Krylov R.A., Ilina L.A., Savenkov K.S., Ponomareva E.S., Zaikin V.A., Laptev G.Y.
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